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羟丙甲基纤维素[HPMC]作为药用辅料在制剂中的应用[6]

文字:[大][中][小] 手机页面二维码 2017-11-27     浏览次数:    
尺寸排阻色谱法是一种常用来测定如水溶性纤维素这种聚合物的分子大小和分子量的方法[8]。分离是基于单个分子的流体力学体积。SEC分析是在配备沃特斯916泵、沃特斯996光电二极管阵列探测器和沃特斯410折光检测器的沃斯特装置上进行的,这种用于多糖的SEC分析的柱子是TOSOHAAS公司的凝胶柱7.8 mm*30 cm.在实验过程中,这个柱子放在恒温35℃的烘箱中。淋洗液为0.05 mol/L的生理盐水(用来防止分子凝聚),可通过去离子水(纯水指数mQ)获得。在实验过程中这种溶液需经过0.22µm和直线脱气过滤。淋洗液流量设定为0.5毫升/分钟。用明确分子量的标准分子进行标定,它的理论分散指数接近于1.根据分子量从5800 到 788000 ,采用SHODEX标准P-82可分为八种聚合物。相应的时间分别是17.4分钟和 12.9 分钟。
HPMC is Size exclusion chromatography is used to determine if such a water-soluble cellulose polymer molecular size and the molecular weight of [8]. Separation is based on hydrodynamic volume of individual molecules. SEC analysis is equipped with a Waters 916 pump, Waters 996 photodiode array detector and a Waters 410 refractive index detector on the Wurster apparatus, this analysis for a polysaccharide SEC column is TOSOHAAS business the gel column 7.8 mm * 30 cm. during the experiment, the temperature in the column oven 35 ℃. Eluent was 0.05 mol / L of normal saline (used to prevent molecular cohesion), through deionized water (pure water index mQ) obtained. During the experiment, the solution was degassed subject to 0.22μm and linear filtering. Eluent flow rate of 0.5 ml / min. Molecular weight molecule with an explicit calibration standards, its theoretical dispersion index close to 1. According to molecular weight from 5800-788000, using the standard P-82 can SHODEX are classified into eight polymer. Respectively corresponding time was 17.4 minutes, and 12.9 minutes.
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